Cryptococcus gattii  is a primary fungal pathogen of humans capable of causing the disease cryptococcosis. Symptoms of cryptococcosis range from asymptomatic pulmonary colonisation, to potentially fatal dissemination to the central nervous system. Molecular techniques divide C. gattii into four genotypes: VGI, VGII, VGIII and VGIV. All four genotypes are capable of causing disease in humans but have some differences in clinical presentation, fertility, epidemiology and response to antifungal drugs. This suggests differences in virulence-related phenotypes may exist among the genotypes. Here, we have collected 88 largely clinical C. gattii isolates from a broad geographic range across the four C. gattii genotypes. Virulence associated phenotypes that we are investigating include thermal tolerance, extracellular phospholipase production, response to oxidative stress, induction of polysaccharide capsule and host immune response. Preliminary data suggest that the genotypes differ in growth and in their response to elevated temperature. These findings underscore the importance of understanding fungal diversity and genotype for understanding and controlling cryptococcal disease.